pHS12-mCherry
(Plasmid #25444)

• Depositing Lab: Benjamin Glick
• Publication: Glick Lab Plasmids (unpublished)

Backbone

• Vector backbone: pHS12
• Backbone size w/o insert (bp): 5000
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mCherry
• Species: Discosoma sp.
• Insert Size (bp): 711
• GenBank ID: AY678264
• Tag / Fusion Protein:
  • COX4 Presequence (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Roger Tsien, UCSD.
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is derived from a construct created by Rob Jensen's group. It encodes mitochondrially-targeted mCherry expressed from the constitutive ADH1 promoter. Cells containing this plasmid have bright red mitochondria with apparently normal morphology.