pEAK10-His-Myc-Kal9
(Plasmid #25441)

• Depositing Lab: Betty Eipper
• Publication: Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-33.

Backbone

• Vector backbone: pEAK-His-Myc
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kal9
• Alt name: Kalirin 9
• Species: R. norvegicus (rat)
• Insert Size (bp): 7700
• Tags / Fusion Proteins:
  • His (N terminal on insert)
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NsiI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: EF-1a Forward
• 3′ sequencing primer: N/A

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are 2 mutations in the sequence, T46S and N62Y. These sequencing errors are the result of mistakes in initial submission to GenBank by the depositing lab. The sequence should be correct.