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Addgene

pEAK10-His-Myc-Kal5
(Plasmid #25440)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25440 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEAK-His-Myc
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kal5
  • Alt name
    Kalirin 5
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    3200
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer EF-1a Forward
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see depositor's sequence for Kal5 for reference.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEAK10-His-Myc-Kal5 was a gift from Betty Eipper (Addgene plasmid # 25440 ; http://n2t.net/addgene:25440 ; RRID:Addgene_25440)

  • For your References section:

    Isoforms of kalirin, a neuronal Dbl family member, generated through use of different 5'- and 3'-ends along with an internal translational initiation site. Johnson RC, Penzes P, Eipper BA, Mains RE. J Biol Chem. 2000 Jun 23. 275(25):19324-33. 10.1074/jbc.M000676200 PubMed 10777487
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