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Addgene

258/-2.5CAT
(Plasmid #25431)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25431 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCATdeltaEH
  • Backbone manufacturer
    Mellon Lab
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression ; Reporter construct

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Myo D Promoter/Enhancer
  • Alt name
    MyoD
  • Alt name
    Myo D
  • Species
    H. sapiens (human)
  • Entrez Gene
    MYOD1 (a.k.a. CMYP17, MYF3, MYOD, MYODRIF, PUM, bHLHc1)
  • Tag / Fusion Protein
    • CAT (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer CAT-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The 258/-2.5 core enhancer/promoter fragment can be removed by digestion with SalI or NotI and XhoI. The resulting fragment is approximately 3kb.

All tk sequences were removed from the CAT vector in the construction of 258/-2.5CAT.

Between the enhancer and promoter is a portion of the pBluescript polylinker tha t includes BamHI, SpeI and SmaI sites, none of which are unique.

258 refers to the core enhancer, which is present in this construct, upstream of the 2.5 kb MyoD promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    258/-2.5CAT was a gift from Charles P. Emerson Jr. (Addgene plasmid # 25431 ; http://n2t.net/addgene:25431 ; RRID:Addgene_25431)

  • For your References section:

    Embryonic activation of the myoD gene is regulated by a highly conserved distal control element. Goldhamer DJ, Brunk BP, Faerman A, King A, Shani M, Emerson CP. Development. 1995 Mar . 121(3):637-49. PubMed 7720572
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