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Addgene

F3/-2.5lacZ
(Plasmid #25430)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25430 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPD46.21
  • Backbone manufacturer
    Fire et al., 1990
  • Backbone size w/o insert (bp) 6110
  • Vector type
    Mammalian Expression ; Reporter construct

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Myo D Promoter
  • Alt name
    MyoD
  • Species
    H. sapiens (human)
  • Entrez Gene
    MYOD1 (a.k.a. CMYP17, MYF3, MYOD, MYODRIF, PUM, bHLHc1)
  • Tag / Fusion Protein
    • lacZ (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer n/A
  • 3′ sequencing primer LacZ-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A SalI-XhoI fragment containing the 2.5 kb promoter fragment was cloned into the SalI site of pPD46.21, destroying the XhoI site. The F3 fragment is cloned in reverse orientation.

258 refers to the core enhancer, which is present in this construct, upstream of the 2.5 kb MyoD promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    F3/-2.5lacZ was a gift from Charles P. Emerson Jr. (Addgene plasmid # 25430 ; http://n2t.net/addgene:25430 ; RRID:Addgene_25430)

  • For your References section:

    Embryonic activation of the myoD gene is regulated by a highly conserved distal control element. Goldhamer DJ, Brunk BP, Faerman A, King A, Shani M, Emerson CP. Development. 1995 Mar . 121(3):637-49. PubMed 7720572
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