pCMV14/3xFLAG-Pax3
(Plasmid #25427)

• Depositing Lab: Jonathan Epstein
• Publication: Epstein Lab Plasmids (unpublished)

Backbone

• Vector backbone: p3XFLAG-CMV-14
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 6310
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Pax3
• Alt name: Pax-3
• Alt name: Paired Box Gene 3
• Species: M. musculus (mouse)
• Insert Size (bp): 1440
• GenBank ID: NT_039173
• Entrez Gene: Pax3 (a.k.a. Pax-3, Sp, Splchl2, splotch)
• Tag / Fusion Protein:
  • 3XFLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The Pax3 insert was derived from pBH3.2 Pax3 plasmid from Martyn Goulding, Max Plank Institute (Goulding MD, Chalepakis G, Deutsch U, Erselius JR, Gruss P. Pax-3, a novel murine DNA binding protein expressed during early neurogenesis. EMBO J. 1991 10(5):1135-47).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry