F3/-2.5CAT
(Plasmid #25425)

• Depositing Lab: Charles P. Emerson Jr.
• Publication: Goldhamer et al Science. 1992 Apr 24. 256(5056):538-42.

Backbone

• Vector backbone: pCATdeltaEH
• Backbone manufacturer: Mellon Lab
• Backbone size w/o insert (bp): 4100
• Vector type: Mammalian Expression ; Reporter construct

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Myo D Promoter + F3
• Alt name: MyoD
• Species: H. sapiens (human)
• Entrez Gene: MYOD1 (a.k.a. CMYP17, MYF3, MYOD, MYODRIF, PUM, bHLHc1)
• Tag / Fusion Protein:
  • CAT (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (destroyed during cloning)
• 3′ cloning site: BglII (destroyed during cloning)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: CAT-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Fragment 3 was cloned into the XbaI site of -2.5CAT by digesting chMD-13 with NotI and EcoRI, and blunt-end-ligating the fragment into the unique XbaI site.

The -2.5 promoter fragment can be removed by digestion with SalI or NotI and XhoI. The resulting fragment is approximately 6.8kb.

All tk sequences were removed from the CAT vector in the construction of -2.5kb.

Between the enhancer and promoter is a portion of pBluescript polylinker that includes BamHI, SpeI and SmaI sites, none of which are unique.