YFP-mDia1FH2deltaN
(Plasmid #25419)

• Depositing Lab: Arthur Alberts
• Publication: Wallar et al Exp Cell Res. 2007 Feb 1. 313(3):560-71.

Backbone

• Vector backbone: YFP-C1
• Backbone manufacturer: clontech Invitrogen
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: diaph1
• Alt name: drf1
• Alt name: mDia1
• Species: M. musculus (mouse)
• Insert Size (bp): 1226
• Mutation: mDia1 FH2∆N2 sequence (encodes amino acids 771-1182)
• GenBank ID: NM_007858
• Entrez Gene: Diaph1 (a.k.a. D18Wsu154e, Dia1, Diap1, Drf1, p140mDia)
• Tag / Fusion Protein:
  • YFP A206K (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl II (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Narumiya Lab Kyoto University

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Also see Copeland, J. W., and R. Treisman. 2002. The Diaphanous-related Formin mDia1 Controls Serum Response Factor Activity through its Effects on Actin Polymerization. Mol Biol Cell 13:4088-99 for information about FH2∆N2 mutation.