pEGFPm-DAD M1041A
(Plasmid #25411)

• Depositing Lab: Arthur Alberts
• Publication: Alberts et al J Biol Chem. 2001 Jan 26. 276(4):2824-30.

Backbone

• Vector backbone: EF-BOS
• Backbone size w/o insert (bp): 3000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Diap3
• Alt name: Diap3 Diaphanous-autoregulatory domain
• Alt name: mDia2 DAD
• Alt name: Diap3 DAD
• Species: M. musculus (mouse)
• Insert Size (bp): 433
• Mutation: Alanine substitution at critical residue in DAD that is required for binding to the Dia-inhibitory domain (DID); the M1041A renders it biologically inactive. Q1091P mutation
• GenBank ID:
• Entrez Gene: Diaph3 (a.k.a. 4930417P13Rik, Dia2, Diap3, Drf3, p134MDia2)
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • Myc (N terminal on backbone)
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GCC ATC TAT TGC TTA CAT TTG CTT CTG ACA CAA CTG
• 3′ sequencing primer: CCA GGG CAT TAG CCA CAC CAG CCA CCA CTT TCT G

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Q1091P mutation in the DAD region was not intentional and may have been a cloning error.