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Addgene

HMGA2-FLAG
(Plasmid #25409)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25409 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMIG
  • Backbone manufacturer
    Gift from David Baltimore
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral ; ChIP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    high mobility group AT-Hook 2
  • Alt name
    HMGA2
  • Alt name
    pygmy
  • Alt name
    Hmgic
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    324
  • Mutation
    None
  • GenBank ID
    NM_010441
  • Entrez Gene
    Hmga2 (a.k.a. 9430083A20Rik, HMGI-C, Hmgic, pg, pygmy)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (unknown if destroyed)
  • 3′ cloning site Bgl2 (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HMGA2-FLAG was a gift from Sean Morrison (Addgene plasmid # 25409 ; http://n2t.net/addgene:25409 ; RRID:Addgene_25409)

  • For your References section:

    Hmga2 promotes neural stem cell self-renewal in young but not old mice by reducing p16Ink4a and p19Arf Expression. Nishino J, Kim I, Chada K, Morrison SJ. Cell. 2008 Oct 17. 135(2):227-39. 10.1016/j.cell.2008.09.017 PubMed 18957199
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