Skip to main content
Addgene

HMGA2 shRNA
(Plasmid #25408)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25408 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pPRIME-CMV-GFP-FF3
  • Backbone manufacturer
    Elledge lab, Addgene plasmid 11663
  • Backbone size w/o insert (bp) 8509
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    high mobility group AT-Hook 2
  • Alt name
    HMGA2
  • Alt name
    pygmy
  • Alt name
    Hmgic
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    97
  • Mutation
    None
  • GenBank ID
    NM_010441
  • Entrez Gene
    Hmga2 (a.k.a. 9430083A20Rik, HMGI-C, Hmgic, pg, pygmy)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (unknown if destroyed)
  • 3′ cloning site EcoR1 (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pPRIME-CMV-GFP-FF3 is available from Addgene.

HMGA2 shRNA sequence:

TGCTGTTGACAGTGAGCGATTTGTGGATCTGATAAGCAAGTAGTGAAGCCACAGATGTACTTGCTTATCAGATCCACAAACTGCCTACTGCCTCGGA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HMGA2 shRNA was a gift from Sean Morrison (Addgene plasmid # 25408 ; http://n2t.net/addgene:25408 ; RRID:Addgene_25408)

  • For your References section:

    Hmga2 promotes neural stem cell self-renewal in young but not old mice by reducing p16Ink4a and p19Arf Expression. Nishino J, Kim I, Chada K, Morrison SJ. Cell. 2008 Oct 17. 135(2):227-39. 10.1016/j.cell.2008.09.017 PubMed 18957199
Related items:
Commonly requested with: