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Addgene

HMGA2+GFP
(Plasmid #25405)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25405 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLentilox RSV
  • Backbone manufacturer
    University of Michigan Vector Core
  • Backbone size w/o insert (bp) 7766
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    high mobility group AT-Hook 2
  • Alt name
    HMGA2
  • Alt name
    pygmy
  • Alt name
    Hmgic
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2933
  • Mutation
    polyadenylation signal lacking
  • GenBank ID
    NM_010441
  • Entrez Gene
    Hmga2 (a.k.a. 9430083A20Rik, HMGI-C, Hmgic, pg, pygmy)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Xba1 (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To view the backbone sequence please visit use the following link:
http://www2.med.umich.edu/medschool/vcore/PlasmidList.cfm

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HMGA2+GFP was a gift from Sean Morrison (Addgene plasmid # 25405 ; http://n2t.net/addgene:25405 ; RRID:Addgene_25405)

  • For your References section:

    Hmga2 promotes neural stem cell self-renewal in young but not old mice by reducing p16Ink4a and p19Arf Expression. Nishino J, Kim I, Chada K, Morrison SJ. Cell. 2008 Oct 17. 135(2):227-39. 10.1016/j.cell.2008.09.017 PubMed 18957199
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