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Addgene

Let-7b-GFP
(Plasmid #25404)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25404 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLentilox 3.7GFP
  • Backbone manufacturer
    University of Michigan Vector Core
  • Backbone size w/o insert (bp) 7650
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mirlet7b
  • Alt name
    Let-7b
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    85
  • Mutation
    None
  • GenBank ID
    NM_029727
  • Entrez Gene
    Mirlet7b (a.k.a. Mirnlet7b, let-7b, mmu-let-7b)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site Xho1 (destroyed during cloning)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The last nucleotide in the Letb insert is G rather than A but will not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Let-7b-GFP was a gift from Sean Morrison (Addgene plasmid # 25404 ; http://n2t.net/addgene:25404 ; RRID:Addgene_25404)

  • For your References section:

    Hmga2 promotes neural stem cell self-renewal in young but not old mice by reducing p16Ink4a and p19Arf Expression. Nishino J, Kim I, Chada K, Morrison SJ. Cell. 2008 Oct 17. 135(2):227-39. 10.1016/j.cell.2008.09.017 PubMed 18957199
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