pPro29b(+)
(Plasmid
#25400)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 25400 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPro29b(+)
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
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Insert Size (bp)5421
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MutationN/A
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Tags
/ Fusion Proteins
- Unknown
- Unknown
- Unknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
https://www.ncbi.nlm.nih.gov/pubmed/18639640
Protein Expr Purif. 2008 Oct;61(2):197-203. Epub 2008 Jun 27.
Heterologous protein production in Escherichia coli using the propionate-inducible pPro system by conventional and auto-induction methods.
Lee SK, Keasling JD.
Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA. [email protected]
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPro29b(+) was a gift from Jay Keasling (Addgene plasmid # 25400 ; http://n2t.net/addgene:25400 ; RRID:Addgene_25400)