pEGFP-mSmo
(Plasmid #25395)

• Depositing Lab: Philip Beachy
• Publication: Chen et al Genes Dev. 2002 Nov 1. 16(21):2743-8.

Backbone

• Vector backbone: pEGFP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mouse Smoothened cDNA
• Alt name: Smo
• Species: M. musculus (mouse)
• Insert Size (bp): 2382
• GenBank ID: NM_176996
• Entrez Gene: Smo (a.k.a. E130215L21Rik, Smoh, bnb, smoothened)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HinD III (unknown if destroyed)
• 3′ cloning site: Sal I (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry