pCMV-Flag-hMcl-1(S159A)
(Plasmid #25393)

• Depositing Lab: Roger Davis
• Publication: pCMV-Flag-hMcl-1 (unpublished)

Backbone

• Vector backbone: pCMV-Tag2A
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MCL1
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• Mutation: changed Serine 159 to Alanine
• GenBank ID: NM_021960
• Entrez Gene: MCL1 (a.k.a. BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bam HI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: CMV Forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

unpublished

There are a few mismatches between the sequence provided and the existing NCBI sequence, specifically - E168 deletion and D173E mutation.
The construct works fine and the mismatches are probably due to outdated reference sequence in NCBI.