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Addgene

pBabe-HA-mMcl-1(T144D)
(Plasmid #25388)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25388 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe IRES puro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mMCL1(T144D)
  • Alt name
    MCL1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1270
  • Mutation
    P142T T144D
  • GenBank ID
    NM_008562
  • Entrez Gene
    Mcl1 (a.k.a. Gm52627, Mcl-1)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal I (not destroyed)
  • 3′ cloning site blunt (destroyed during cloning)
  • 5′ sequencing primer pBabe 5'
  • 3′ sequencing primer pBabe 3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-HA-mMcl-1(T144D) was a gift from Roger Davis (Addgene plasmid # 25388 ; http://n2t.net/addgene:25388 ; RRID:Addgene_25388)

  • For your References section:

    Mcl-1 integrates the opposing actions of signaling pathways that mediate survival and apoptosis. Morel C, Carlson SM, White FM, Davis RJ. Mol Cell Biol. 2009 Jul . 29(14):3845-52. 10.1128/MCB.00279-09 PubMed 19433446
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