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Addgene

pCDNA3-HA-mMcl1(S140A)
(Plasmid #25383)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25383 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mMCL1(S140A)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1270
  • Mutation
    changed serine 140 to alanine
  • GenBank ID
    NM_008562
  • Entrez Gene
    Mcl1 (a.k.a. Gm52627, Mcl-1)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3-HA-mMcl1(S140A) was a gift from Roger Davis (Addgene plasmid # 25383 ; http://n2t.net/addgene:25383 ; RRID:Addgene_25383)

  • For your References section:

    Mcl-1 integrates the opposing actions of signaling pathways that mediate survival and apoptosis. Morel C, Carlson SM, White FM, Davis RJ. Mol Cell Biol. 2009 Jul . 29(14):3845-52. 10.1128/MCB.00279-09 PubMed 19433446
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