pBabe-Flag hMcl-1(T163A)
(Plasmid #25373)

• Depositing Lab: Roger Davis
• Publication: Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52.

Backbone

• Vector backbone: pBabe IRES puro
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hMCL1(T163A)
• Alt name: MCL1
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• Mutation: changed threonine 163 to alanine
• GenBank ID: NM_021960
• Entrez Gene: MCL1 (a.k.a. BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: blunt (destroyed during cloning)
• 3′ cloning site: Sal I (not destroyed)
• 5′ sequencing primer: pBabe 5'
• 3′ sequencing primer: pBabe 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are a few mismatches between the sequence provided and the existing NCBI sequence, specifically - E168 deletion and D173E mutation.
The construct works fine and the mismatches are probably due to outdated reference sequence in NCBI.