2XMyc-LRRK2-3XKD
(Plasmid #25366)

• Depositing Lab: Mark Cookson
• Publication: J Biol Chem. 2008 Jun 13;283(24):16906-14.

Backbone

• Vector backbone: pCMV-Tag 3B
• Backbone manufacturer: Cookson Lab
• Backbone size w/o insert (bp): 4300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LRRK2
• Species: H. sapiens (human)
• Insert Size (bp): 7581
• Mutation: "Kinase Dead" Lysine 1906 mutated to Alanine. Aspartate 1994 mutated to Alanine. Aspartate 2017 mutated to Alanine.
• Entrez Gene: LRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
• Tag / Fusion Protein:
  • 2XMyc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • Diagnostic Digest for 2XMyc-LRRK2 plasmids
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

An additional Myc tag has been added to the original vector. The 2XMyc vector was adapted to the invitrogen gateway system by insertion of a gateway cloning cassette. LRRK2 was cloned by gateway recombination.

In order to provide these LRRK2 plasmids as a resource to the community, we depend on the contributing academic laboratory for quality control. Therefore, the full-length LRRK2 plasmids are distributed in the format provided by the contributing institution "as is" with no additional guarantee.
Addgene NGS detected additional S212L and I1122V mutations in this plasmid relative to AAV63975.1.
Addgene is not responsible for any errors in these constructs.

It is strongly encouraged that a diagnostic digest be performed on this plasmid before use in an experiment (see attached PDF).

If there is an issue with your stock, please contact the Cookson lab for a replacement.