2XMyc-LRRK2-WT Notes
Addgene diagnostic digest
This gel depicts the NEB 1kb extend DNA ladder and a digestion of plasmid 25361 with BamHI-HF, SalI-HF, and NdeI.
Our restriction digest shows the expected banding patterns:
BamHI-HF single: 1 band @ 12KB
SalI-HF single: 1 band @ 12KB
BamHI-HF / SalI-HF double: 2 bands - 7.4KB and 4.5KB
NdeI single: 2 bands - 8.5KB and 3.5KB
