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Addgene

UHRF1
(Plasmid #25359)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 25359 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET28a-LIC
  • Backbone manufacturer
    SGC
  • Backbone size w/o insert (bp) 7328
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ubh12.672.793
  • Alt name
    PDB: 3FL2
  • Alt name
    UHRF1, RING domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    366
  • Mutation
    amino acid residues 672-793.
  • GenBank ID
    NP_037414
  • Entrez Gene
    UHRF1 (a.k.a. ICBP90, Np95, RNF106, TDRD22, hNP95, hUHRF1, huNp95)
  • Tag / Fusion Protein
    • His with thrombin cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UHRF1 was a gift from Cheryl Arrowsmith (Addgene plasmid # 25359 ; http://n2t.net/addgene:25359 ; RRID:Addgene_25359)
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