RNF128
(Plasmid
#25356)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 25356 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepFHMSP-LIC-N
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Backbone manufacturerSGC
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRNF128.038.204
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Alt namePDB: 3ICU
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Alt nameE3 Ligase GRAIL, PA domain
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Alt nameRNF128
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SpeciesH. sapiens (human)
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Insert Size (bp)501
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GenBank IDBC063404
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Entrez GeneRNF128 (a.k.a. GRAIL, FLJ23516)
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Tag
/ Fusion Protein
- His (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PDB: 3ICU. http://www.thesgc.org/structures/3ICU/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RNF128 was a gift from Cheryl Arrowsmith (Addgene plasmid # 25356 ; http://n2t.net/addgene:25356 ; RRID:Addgene_25356)