DNMT3A
(Plasmid #25328)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pET28-MHL
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7325
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: DNMT3A_11
• Alt name: PDB: 3LLR
• Alt name: DNA (cytosine-5-)-methyltransferase 3 alpha, PWWP domain
• Alt name: DNMT3A
• Species: H. sapiens (human)
• Insert Size (bp): 450
• Entrez Gene: DNMT3A (a.k.a. DNMT3A2, HESJAS, M.HsaIIIA, TBRS)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 3LLR. http://www.thesgc.org/structures/3LLR/