WDR39
(Plasmid #25308)

• Purpose: Expresses His-tagged WDR39 (CIAO1) in bacteria
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pET28a-LIC
• Backbone manufacturer: SGC (Addgene Plasmid #26094)
• Backbone size w/o insert (bp): 7328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: WDR39_1
• Alt name: PDB: 3FM0
• Alt name: WD40 protein CIAO1
• Alt name: WDR39
• Species: H. sapiens (human)
• Insert Size (bp): 1017
• GenBank ID: NP_004795
• Entrez Gene: CIAO1 (a.k.a. CIA1, WDR39)
• Promoter: T7-lacO
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 3FM0. http://www.thesgc.org/structures/3FM0/