HDAC6 (3GV4)
(Plasmid #25297)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pET28a-LIC
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HDAC6_42
• Alt name: HDAC6 zinc finger domain
• Alt name: 3GV4, 5KH3, 5KH7
• Alt name: 5KH9, 5B8D, 3C5K
• Species: H. sapiens (human)
• Insert Size (bp): 321
• Mutation: Contains amino acid residues 1109-1215
• GenBank ID: BC013737
• Entrez Gene: HDAC6 (a.k.a. CPBHM, HD6, JM21, KDAC6, PPP1R90)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB / SGC links:
3GV4 / http://www.thesgc.org/structures/3GV4/
5KH3 / http://www.thesgc.org/structures/5KH3/
5KH7 / http://www.thesgc.org/structures/5KH7/
5KH9 / http://www.thesgc.org/structures/5KH9/
5B8D / http://www.thesgc.org/structures/5B8D/
3C5K / http://www.thesgc.org/structures/3C5K/