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Addgene

NRASA
(Plasmid #25256)

Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 25256 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pET28-MHL
  • Backbone manufacturer
    SGC
  • Backbone size w/o insert (bp) 7325
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NRASA:M1-N172
  • Alt name
    PDB: 3CON
  • Alt name
    neuroblastoma RAS viral (v-ras) oncogene homolog
  • Alt name
    NRASA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    516
  • Mutation
    Contains amino acids M1-N172
  • Entrez Gene
    NRAS (a.k.a. ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6)
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NRASA was a gift from Cheryl Arrowsmith (Addgene plasmid # 25256 ; http://n2t.net/addgene:25256 ; RRID:Addgene_25256)