CBX3 (3DM1)
(Plasmid #25242)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pET28-MHL
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7325
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CBX3_05
• Alt name: PDB: 3DM1
• Alt name: chromobox homolog 3
• Alt name: CBX3
• Species: H. sapiens (human)
• Insert Size (bp): 174
• Entrez Gene: CBX3 (a.k.a. HECH, HP1-GAMMA, HP1Hs-gamma, HP1gamma)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 3DM1. http://www.thesgc.org/structures/3DM1/