SETDB1
(Plasmid
#25240)
-
PurposeBacterial expression for structure determination; may not be full ORF
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 25240 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
* Log in to view industry pricing.
Backbone
-
Vector backbonepET28-MHL
-
Backbone manufacturerSGC
- Backbone size w/o insert (bp) 7325
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameSETDB1, SETDB1_16
-
Alt namehistone-lysine N-2 methyltransferase (SET domain bifurcated 1), Tudor domain
-
Alt nameSETDB1:APC043-C09:C27644
-
Alt namePDB IDs: 3DLM, 8G5E, 8UWP
-
SpeciesH. sapiens (human)
-
Insert Size (bp)621
-
Entrez GeneSETDB1 (a.k.a. ESET, H3-K9-HMTase4, KG1T, KMT1E, TDRD21)
- Promoter T7
-
Tags
/ Fusion Proteins
- 6xHis-TEV (N terminal on insert)
- MHHHHHHSSGRENLYFQG (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For more information, please see:
PDB / SGC
http://www.thesgc.org/structures/3DLM/
https://www.rcsb.org/structure/8G5E/
https://www.rcsb.org/structure/8UWP/
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
SETDB1 was a gift from Cheryl Arrowsmith (Addgene plasmid # 25240 ; http://n2t.net/addgene:25240 ; RRID:Addgene_25240)