CYP2R1 (2OJD)
(Plasmid #25236)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pCW-LIC
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 6980
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CYP2R1_cw_01
• Alt name: PDB: 2OJD, 3CZH, 3DL9
• Alt name: cytochrome P450, family 2, R1
• Alt name: CYPR1
• Species: H. sapiens (human)
• Insert Size (bp): 1437
• Mutation: Contains amino acids 32-501
• Entrez Gene: CYP2R1
• Tag / Fusion Protein:
  • 4xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pCW-SEQ-Fwd
• 3′ sequencing primer: pCW-SEW-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB links:
http://www.thesgc.org/structures/2OJD/
http://www.thesgc.org/structures/3CZH/
http://www.thesgc.org/structures/3DL9/