UBH12 (3BI7)
(Plasmid #25219)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pNIC-CH
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7218
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ubh12.414.617
• Alt name: PDB: 3BI7, 6VCS
• Alt name: UHRF1, SRA domain
• Species: H. sapiens (human)
• Insert Size (bp): 612
• Mutation: SRA Domain: Residues 414-617
• Entrez Gene: UHRF1 (a.k.a. ICBP90, Np95, RNF106, TDRD22, hNP95, hUHRF1, huNp95)
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pLIC-For
• 3′ sequencing primer: pLIC-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: http://www.thesgc.org/structures/3BI7/
https://www.thesgc.org/structures/6VCS/