MALT1 (3BFO)
(Plasmid #25216)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pET28a-LIC
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: malt1.226.314
• Alt name: PDB: 3BFO
• Alt name: Malt1
• Alt name: mucosa associated lymphoid tissue lymphoma translocation gene 1
• Species: H. sapiens (human)
• Insert Size (bp): 267
• Mutation: Contains amino acid residues 226-314 of Isoform 2
• Entrez Gene: MALT1 (a.k.a. IMD12, MLT, MLT1, PCASP1)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 3BFO. http://www.thesgc.org/structures/3BFO/