PPT1
(Plasmid #25205)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pFHMSP-LIC-C
• Backbone manufacturer: SGC
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PPT1_02
• Alt name: PDB: 3GRO
• Alt name: palmitoyl-protein thioesterase 1
• Alt name: PPT1
• Species: H. sapiens (human)
• Insert Size (bp): 855
• Entrez Gene: PPT1 (a.k.a. CLN1, INCL, PPT)
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 3GRO. http://www.thesgc.org/structures/3GRO/