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Addgene

NR1D2
(Plasmid #25186)

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Item Catalog # Description Quantity Price (USD)
Plasmid 25186 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pFBOH-LIC
  • Backbone manufacturer
    SGC
  • Backbone size w/o insert (bp) 6770
  • Vector type
    Baculovirus expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NR1D2_07
  • Alt name
    PDB: 3CQV
  • Alt name
    nuclear receptor subfamily 1, group D, member 2
  • Alt name
    NR1D2, BD73, EAR-1r, Hs.37288, HZF2, RVR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    768
  • GenBank ID
    NP_005117
  • Entrez Gene
    NR1D2 (a.k.a. BD73, EAR-1R, REVERBB, REVERBbeta, RVR)
  • Promoter polyhedrin
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-Fwd
  • 3′ sequencing primer pFBOH-Rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NR1D2 was a gift from Cheryl Arrowsmith (Addgene plasmid # 25186 ; http://n2t.net/addgene:25186 ; RRID:Addgene_25186)
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