NEDD5
(Plasmid #25184)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pET28a-LIC
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NEDD5:E13-Q320-B3_W260A
• Alt name: PDB: 2QNR
• Alt name: Septin 2, GTPase domain
• Alt name: NEDD5
• Species: H. sapiens (human)
• Insert Size (bp): 924
• Mutation: Contains amino acids E13-Q320. W260A mutation.
• Entrez Gene: SEPTIN2 (a.k.a. DIFF6, NEDD-5, NEDD5, Pnutl3, SEPT2, hNedd5)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 2QNR. http://www.thesgc.org/structures/2QNR/