NEDD4L
(Plasmid #25169)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pET28-MHL
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7325
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: nedd4l.0574.0947
• Alt name: PDB: 2ONI
• Alt name: ubiquitin-protein ligase NEDD4-like catalytic domain
• Species: H. sapiens (human)
• Insert Size (bp): 1122
• Mutation: Hect Domain
• Entrez Gene: NEDD4L (a.k.a. NEDD4-2, NEDD4.2, PVNH7, RSP5, hNEDD4-2)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 2ONI. http://www.thesgc.org/structures/2ONI/