CYP51A1 (3I3K)
(Plasmid
#25161)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 25161 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepCW-LIC
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Backbone manufacturerSGC
- Backbone size w/o insert (bp) 6980
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCYP51A1_cw_09
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Alt namePDB ID: 3LD6 (supersedes 3I3K)
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Alt namelanosterol 14alpha-demethylase (CYP51)
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SpeciesH. sapiens (human)
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Insert Size (bp)1377
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MutationContains amino acid residues 54-502
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Entrez GeneCYP51A1 (a.k.a. CP51, CYP51, CYPL1, LDM, P450-14DM, P450L1)
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pCW-SEQ-fwd
- 3′ sequencing primer pCW-SEQ-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PDB ID: 3LD6 (supersedes 3I3K) https://www.rcsb.org/structure/3LD6
SGC link: https://www.thesgc.org/structures/3I3K/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CYP51A1 (3I3K) was a gift from Cheryl Arrowsmith (Addgene plasmid # 25161 ; http://n2t.net/addgene:25161 ; RRID:Addgene_25161)