CBX2
(Plasmid #25158)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pET28-MHL
• Backbone manufacturer: SGC (Addgene Plasmid # 26096)
• Backbone size w/o insert (bp): 7325
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CBX2_04
• Alt name: PDB IDs: 3H91, 5EPK
• Alt name: chromobox homolog 2
• Species: H. sapiens (human)
• Insert Size (bp): 162
• Entrez Gene: CBX2 (a.k.a. CDCA6, M33, SRXY5)
• Promoter: T7
• Tags / Fusion Proteins:
  • 6x His (N terminal on backbone)
  • TEV cleavage site (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

For more information, please see:
http://www.thesgc.org/structures/3H91/
http://www.thesgc.org/structures/5EPK/