UBC64
(Plasmid
#25143)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 25143 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepET28a-LIC
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Backbone manufacturerSGC
- Backbone size w/o insert (bp) 7328
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameubc64.248.414
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Alt namePDB: 2QGX
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Alt nameubiquitin-conjugating enzyme E2Q
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SpeciesH. sapiens (human)
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Insert Size (bp)501
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Mutationcontains UBC domain; residues 248-414
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Entrez GeneUBE2Q1 (a.k.a. GTAP, NICE-5, PRO3094, UBE2Q)
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Tag
/ Fusion Protein
- His tag with thrombin cleavage (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PDB: 2QGX. http://www.thesgc.org/structures/2QGX/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UBC64 was a gift from Cheryl Arrowsmith (Addgene plasmid # 25143 ; http://n2t.net/addgene:25143 ; RRID:Addgene_25143)