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pcDNA3flag-RanGAPdeltaN
(Plasmid #25104)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25104 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RanGAP1 deleted N-term domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    601
  • Mutation
    Deleted amino acids 1-203.
  • GenBank ID
    AK303524
  • Entrez Gene
    RANGAP1 (a.k.a. Fug1, RANGAP, SD)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6 or BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3flag-RanGAPdeltaN was a gift from Guy Salvesen (Addgene plasmid # 25104 ; http://n2t.net/addgene:25104 ; RRID:Addgene_25104)

  • For your References section:

    Small ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs. Mikolajczyk J, Drag M, Bekes M, Cao JT, Ronai Z, Salvesen GS. J Biol Chem. 2007 Sep 7. 282(36):26217-24. 10.1074/jbc.M702444200 PubMed 17591783
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