pET28a-pro-sumo1
(Plasmid #25101)

• Depositing Lab: Guy Salvesen
• Publication: Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-24.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha for plasmid purification. BL21(DE3) for protein production.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pro-SUMO1
• Alt name: Small ubiquitin-related modifier 1
• Alt name: Sentrin
• Alt name: Ubiquitin-like protein SMT3C
• Species: H. sapiens (human)
• Insert Size (bp): 330
• Mutation: Changed Serine 2 to Alanine
• GenBank ID: U83117
• Entrez Gene: SUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry