pJT104
(Plasmid
#25088)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 25088 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSB4A3
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Backbone manufacturerBioBrick vector-Shetty et al 2008
- Backbone size w/o insert (bp) 3443
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEdge detector delta luxI
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SpeciesE.coli
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Insert Size (bp)5317
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene's quality control sequence detected a 1bp mut in LacZ ORF; it is not known to affect function
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJT104 was a gift from Christopher Voigt (Addgene plasmid # 25088 ; http://n2t.net/addgene:25088 ; RRID:Addgene_25088) -
For your References section:
A synthetic genetic edge detection program. Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt CA, Ellington AD. Cell. 2009 Jun 26. 137(7):1272-81. 10.1016/j.cell.2009.04.048 PubMed 19563759