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Addgene

pBS-hsFlt3L
(Plasmid #25074)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25074 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS
  • Backbone size w/o insert (bp) 2961
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human soluble FMS-like tyrosine kinase 3 ligand
  • Alt name
    Flt3L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1078
  • Entrez Gene
    FLT3LG (a.k.a. FL, FLG3L, FLT3L)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-hsFlt3L was a gift from Maria Castro (Addgene plasmid # 25074 ; http://n2t.net/addgene:25074 ; RRID:Addgene_25074)

  • For your References section:

    Fms-like tyrosine kinase 3 ligand recruits plasmacytoid dendritic cells to the brain. Curtin JF, King GD, Barcia C, Liu C, Hubert FX, Guillonneau C, Josien R, Anegon I, Lowenstein PR, Castro MG. J Immunol. 2006 Mar 15. 176(6):3566-77. PubMed 16517725
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