2XMyc-LRRK2-deltaHeat
(Plasmid #25068)

• Depositing Lab: Mark Cookson
• Publication: J Biol Chem. 2008 Jun 13;283(24):16906-14.

Backbone

• Vector backbone: pCMV-Tag 3B
• Backbone manufacturer: Cookson Lab
• Backbone size w/o insert (bp): 4300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LRRK2
• Species: H. sapiens (human)
• Insert Size (bp): 4746
• Mutation: Contains the LRR-ROC-COR-Kinase-WD40 domains; Deletion of aa 1-795
• Entrez Gene: LRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
• Tag / Fusion Protein:
  • 2XMyc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

An additional Myc tag has been added to the original vector. The 2XMyc vector was adapted to the invitrogen gateway system by insertion of a gateway cloning cassette. LRRK2 was cloned by gateway recombination.

The domain structure and amino acid limits is approximate. According to depositor's domain organization, this construct contains the correct residues.