2XMyc-LRRK2-RCK-Y1699C
(Plasmid
#25067)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 25067 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV-Tag 3B
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Backbone manufacturerCookson Lab
- Backbone size w/o insert (bp) 4300
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLRRK2
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SpeciesH. sapiens (human)
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Insert Size (bp)2670
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MutationContains only the ROC-COR-Kinase domains aa 1329-2219 Tyrosine 1699 mutated to Cysteine
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Entrez GeneLRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
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Tag
/ Fusion Protein
- 2XMyc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
An additional Myc tag has been added to the original vector. The 2XMyc vector was adapted to the invitrogen gateway system by insertion of a gateway cloning cassette. LRRK2 was cloned by gateway recombination.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
2XMyc-LRRK2-RCK-Y1699C was a gift from Mark Cookson (Addgene plasmid # 25067 ; http://n2t.net/addgene:25067 ; RRID:Addgene_25067) -
For your References section:
The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation. . J Biol Chem. 2008 Jun 13;283(24):16906-14. 10.1074/jbc.M708718200 PubMed 18397888
Map uploaded by the depositor.
