pGSTag BimL(AAA)
(Plasmid #25063)

• Depositing Lab: Roger Davis
• Publication: Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.

Backbone

• Vector backbone: pGSTag
• Backbone size w/o insert (bp): 5057
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BimL (AAA)
• Alt name: BCL2-like 11
• Species: H. sapiens (human)
• Insert Size (bp): 257
• Mutation: Changed serine 44 to alanine Changed threonine 56 to alanine Changed serine 58 to alanine
• Entrez Gene: BCL2L11 (a.k.a. BAM, BIM, BOD)
• Tag / Fusion Protein:
  • Gst (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bam HI (not destroyed)
• 3′ cloning site: Hind III (not destroyed)
• 5′ sequencing primer: pGex 5'
• 3′ sequencing primer: pGex 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry