pCDNA3 Flag BimL(T56D,DE)
(Plasmid
#25060)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 25060 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 5446
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBimL (T56D,DE)
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Alt nameBCL2-like 11
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SpeciesH. sapiens (human)
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Insert Size (bp)458
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MutationChanged threonine 56 to aspartic acid changed leucine 92 to aspartic acid changed isoleucine 95 to glutamic acid
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GenBank IDAF032458
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Entrez GeneBCL2L11 (a.k.a. BAM, BIM, BOD)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hind III (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3 Flag BimL(T56D,DE) was a gift from Roger Davis (Addgene plasmid # 25060 ; http://n2t.net/addgene:25060 ; RRID:Addgene_25060) -
For your References section:
JNK phosphorylation of Bim-related members of the Bcl2 family induces Bax-dependent apoptosis. Lei K, Davis RJ. Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7. 10.1073/pnas.0438011100 PubMed 12591950