pCDNA3 Flag BimL(T56D,DE)
(Plasmid #25060)

• Depositing Lab: Roger Davis
• Publication: Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 100(5):2432-7.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BimL (T56D,DE)
• Alt name: BCL2-like 11
• Species: H. sapiens (human)
• Insert Size (bp): 458
• Mutation: Changed threonine 56 to aspartic acid changed leucine 92 to aspartic acid changed isoleucine 95 to glutamic acid
• GenBank ID: AF032458
• Entrez Gene: BCL2L11 (a.k.a. BAM, BIM, BOD)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry