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Addgene

pDEST53-LRRK2-3XKD
(Plasmid #25049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25049 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA-DEST53
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7767
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LRRK2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7584
  • Mutation
    "Kinase Dead" Lysine 1906 mutated to Alanine. Aspartate 1994 mutated to Alanine. Aspartate 2017 mutated to Alanine.
  • Entrez Gene
    LRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR1 (not destroyed)
  • 3′ cloning site attR2 (not destroyed)
  • 5′ sequencing primer CMV-F, GFP-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST53-LRRK2-3XKD was a gift from Mark Cookson (Addgene plasmid # 25049 ; http://n2t.net/addgene:25049 ; RRID:Addgene_25049)

  • For your References section:

    Kinase activity is required for the toxic effects of mutant LRRK2/dardarin. Greggio E, Jain S, Kingsbury A, Bandopadhyay R, Lewis P, Kaganovich A, van der Brug MP, Beilina A, Blackinton J, Thomas KJ, Ahmad R, Miller DW, Kesavapany S, Singleton A, Lees A, Harvey RJ, Harvey K, Cookson MR. Neurobiol Dis. 2006 Aug . 23(2):329-41. 10.1016/j.nbd.2006.04.001 PubMed 16750377
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