pDEST53-LRRK2-WT Notes
Addgene Diagnostic Digest- EcoRI
This gel depicts the NEB 1kb ladder and a digestion of the plasmid pDEST53-LRRK2-WT with the following enzymes: EcoRI-HF.
Expected Bands
EcoRI-HF: 6142 bp, 3404 bp, 2198 bp, 1200 bp & 805bp
We observe the expected bands for this digest.
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ext Ladder and a digestion of the plasmid pDEST53-LRRK2-WT with the following enzyme: EcoRV-HF
Expected bands:
EcoRV-HF: 10.5 + 3.3 kb (if wrong, may have extra band at ~4kb)
Results:
We observed the expected bands for this digest.