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Addgene

pDEST53-LRRK2-WT Notes

Addgene Diagnostic Digest- EcoRI

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pDEST53-LRRK2-WT with the following enzymes: EcoRI-HF.

Expected Bands
EcoRI-HF: 6142 bp, 3404 bp, 2198 bp, 1200 bp & 805bp

We observe the expected bands for this digest.

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ext Ladder and a digestion of the plasmid pDEST53-LRRK2-WT with the following enzyme: EcoRV-HF

Expected bands:
EcoRV-HF: 10.5 + 3.3 kb (if wrong, may have extra band at ~4kb)

Results:
We observed the expected bands for this digest.