pMIR-CDH1 mutUTR
(Plasmid #25039)

• Depositing Lab: Bob Weinberg
• Publication: Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Backbone

• Vector backbone: pMIR-report
• Backbone manufacturer: Ambion
• Backbone size w/o insert (bp): 6470
• Vector type: Mammalian Expression, Luciferase
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CDH1 mutant 3'UTR
• Alt name: CDH1
• Species: H. sapiens (human)
• Insert Size (bp): 819
• Mutation: substitution of 6nt within the miR-9 binding site from CAAAGA to GTTTCT.
• Entrez Gene: CDH1 (a.k.a. Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: Luc-F
• 3′ sequencing primer: M13/pUC Forward

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A partial CDH1 3'UTR (819bp) was PCR-amplified from normal human genomic DNA and cloned into the pMIR-report vector. The mutant construct of the CDH1 3′ UTR was generated with a QuikChange Site-Directed Mutagenesis Kit (Stratagene).